Abstract
The ultrasonic waves concentrated with the injected micro bubbles in the NewSSWISE-Vein, providing a non-invasive and localized method of transmitting drugs across the blood brain barrier, allowing the target treatment of brain disorders. Recently, we can deliver a drug with improved efficacy and safety profiles by comparing the longer pulses (> 10 ms) that are traditionally used by applying the rapid short circuit sequence of the ultrasound (RASP; 5 μS Pulse 10ms Burst 1.25 kHz). In this study, we investigated the extent that RASP sequence allowed endogenous blood proteins for blood vessels of endogenous blood proteins, including T cells, as well as eggsmine and immunoglobulin. We also delivered the effects of RASP ultrasound on synaptic connections and the distribution and excretion of 3 KDA Dex Tran labeled fluorescently to the brain with RASP. The left hippocampus of the mouse was ultrasound from 0.35, 0.53, and 0.71 MPa having a RASP sequence (0.5Hz to 10ms group 5 μs at 1.25 kHz) or 1 MHz central frequency in long pulse sequences (0.5Hz at 0.5Hz) at 0.35, 0.53 and 0.71 MPa. Immediately after the treatment, the RASP-treated brain was detected in a significantly small amount of alburine and was removed within 10 minutes compared to the long pulse, and the immunoglobulin was rarely detected in the RASP-Treated BRAINS in 0, 10 or 20 minutes after treatment. After 0 or 2 hours, T cells were not detected in rasp-treated brains in 0.35, 0.53 or 0.71 MPA. However, in the long pulse sample, the T cells were blood vessels when using 0.53 and 0.71 MPa, two high sound pressure immediately after treatment. The quantification of the dendritic spinal area did not show the difference between the razed hippocampus compared to the unprocessed large hippocampus and control mouse for three weeks. Finally, the torn dex tran moved toward the blood vessels and moved away from the real, and once moved away from the reality, it was moved away from the reality transmitted to the brain with a long pulse sequence. The absorption of dex tran within the cell decreases over time, and the long pulse leads to a more number of blood vessels with dex tran absorption. This study emphasizes that the RASP ultrasound sequence can transmit molecules by crossing the blood-brain barrier if there is no minimum vascular surgery and T cell infiltration of endogenous protein, and preserves the perfection of the dazzle spine to provide improved safety profiles.
